Surface electrophoresis of ds-DNA across orthogonal pair of surfaces

A Ghosh and TK Patra and R Kant and RK Singh and JK Singh and S Bhattacharya, APPLIED PHYSICS LETTERS, 98, 164102 (2011).

DOI: 10.1063/1.3565238

A gel free microchannel device made up of polydimethyl siloxane is fabricated for the surface based electrophoresis of double stranded deoxy-ribonucleic acid (DNA) molecules. In the presence of directional external electric field, DNA fragments near the corners of the microchannel are found to separate faster as compared to those over the base of the channel. This is in spite of the reduction in the mobility of molecules over the channel corners. We performed coarse grained molecular dynamics simulations which reveal that, though the adsorption energy of the DNA fragments increases near the corner, it is the increase in the relative mobility which enhances the separation of the fragments over the corner. (c) 2011 American Institute of Physics. doi: 10.1063/1.3565238

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