Kinetic Monte Carlo and cellular particle dynamics simulations of multicellular systems

E Flenner and L Janosi and B Barz and A Neagu and G Forgacs and I Kosztin, PHYSICAL REVIEW E, 85, 031907 (2012).

DOI: 10.1103/PhysRevE.85.031907

Computer modeling of multicellular systems has been a valuable tool for interpreting and guiding in vitro experiments relevant to embryonic morphogenesis, tumor growth, angiogenesis and, lately, structure formation following the printing of cell aggregates as bioink particles. Here we formulate two computer simulation methods: (1) a kinetic Monte Carlo (KMC) and (2) a cellular particle dynamics (CPD) method, which are capable of describing and predicting the shape evolution in time of three-dimensional multicellular systems during their biomechanical relaxation. Our work is motivated by the need of developing quantitative methods for optimizing postprinting structure formation in bioprinting- assisted tissue engineering. The KMC and CPD model parameters are determined and calibrated by using an original computational- theoretical-experimental framework applied to the fusion of two spherical cell aggregates. The two methods are used to predict the (1) formation of a toroidal structure through fusion of spherical aggregates and (2) cell sorting within an aggregate formed by two types of cells with different adhesivities.

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